What is a MRC-5 cell?

MRC-5 cells are human fetal lung fibroblast cells. MRC-5 cells were derived from embryonic lung tissue of 14-week-old Caucasian male fetus in 1966. These diploid cells have been used extensively in vaccine production and other research.

Where do VERO cells come from?

Vero cells are derived from the kidney of an African green monkey, and are one of the more commonly used mammalian continuous cell lines in microbiology, and molecular and cell biology research.

What are human diploid cells?

Diploid is a cell or organism that has paired chromosomes, one from each parent. In humans, cells other than human sex cells, are diploid and have 23 pairs of chromosomes. Human sex cells (egg and sperm cells) contain a single set of chromosomes and are known as haploid.

Why is it called WI-38?

The cell line in this case is called WI-38, in which the initials represent the Wistar Institute of Anatomy and Biology in Philadelphia, Pennsylvania, where the early work was done.

Is MRC-5 immortalized?

Therefore, the MRC-5hTERT cells can be designated as immortalized, as has been previously reported (13,27).

What is WI-38 in vaccines?

The WI-38 cell line is used as a culture to grow live viruses that are used in vaccines. “Merck, as well as other vaccine manufacturers, uses two well-established human cell lines to grow the virus for selected vaccines,” Merck said in a statement to ABC News.

What do Vero cells do?

Uses in research as host cells for growing viruses; for example, to measure replication in the presence or absence of a research pharmaceutical, the testing for the presence of rabies virus, or the growth of viral stocks for research purposes.

Should we use Vero cell?

Vero cell lines are used less frequently in biological studies than the more popular HeLa cell line, in part because this is a non-human cell line. However, Vero cell lineages are still widely used for screening purposes for bacterial toxins, viruses and for parasite studies.

What is a diploid cell example?

Examples of diploid cells include blood cells, skin cells and muscle cells. These cells are known as somatic cells.

What are WI-38 cells?

The WI-38 cell line is the first human diploid cell line to be used in human vaccine preparation. WI-38 cells are isolated from the lung tissue of a 3-month-old, female, embryo. They can be used as a transfection host and in viruscide testing.

What are Wiwi-·38 cells?

WI-·38 cells the first normal human cells, derived from fetal lung tissue, continuously cultivated. [Wistar Institute] Farlex Partner Medical Dictionary © Farlex 2012 Want to thank TFD for its existence? Tell a friend about us, add a link to this page, or visit the webmaster’s page for free fun content.

What is the karyotype of WI-38?

The WI-38 cell line was developed in July 1962 from lung tissue taken from a therapeutically aborted fetus of about 3 months gestational age. Cells released by trypsin digestion of the lung tissue were used for the primary culture. The cell morphology is fibroblast-like. The karyotype is 46,XX; normal diploid female.

What do we know about DNA synthesis in WI-38 cells?

When normal human diploid fibroblasts (WI-38) are stimulated to proliferate, the earliest detectable DNA synthesis is in the form of small 4.5 S pieces. These “Okazaki fragments” are converted, after about 10 hr, into large chromosomal-sized DNA of 400 S*. We have detected similar small pieces of DNA in transformed WI-38 cells (called 2RA).