How do you store columns in chromatography?

Silica-based normal phase columns should be stored with heptane or another inert solvent. Reversed phase columns are best stored in mixtures of organic compounds (acetonitril, methanol) and water, where the water content must not be higher than 50%.

What is ACE column?

ACE HPLC columns are designed to meet even the most challenging of chromatographic applications, giving excellent performance with acidic, basic and neutral molecules. A wide range of particle sizes, pore sizes, bonded chemistries and column dimensions are available.

What is Ace C18 column?

ACE C18-AR columns are manufactured from the same ultra inert, high purity silica as all ACE phases – ensuring excellent chromatography and excellent reproducibility are obtained.

How do I preserve a column in HPLC?

HPLC Column Care & Storage

1. Routinely monitor the column’s performance.
2. Switch only between miscible mobile phases.
3. Avoid precipitating salts in the column.
4. Use filtered and degassed mobile phases.
5. Do not allow the column to dry out.
6. For prolonged storage, use a mobile phase that inhibits bacterial and mold growth.

How do you store columns?

Normal phase columns should be stored in Isooctane/Ethanol. Reversed phase stationary phases should be kept in a mixture of water / organic modifier (Acetonitrile/Methanol), free from any buffers or additives that might precipitate during storage.

What is column storage in HPLC?

Storage of HPLC columns For short-term storage, the mobile phase can remain in the column. Alternatively, the mobile phase may pass through at very low flow rate. For storage over several days, a buffer-containing eluent is recommended to rinse the column with water to prevent microbial growth.

How long do HPLC columns last?

Normal phase column have slightly shorter life spans, but should accept about 1000 injections. When subjected to extreme conditions, most columns will be stable for only a few hundred injections or less. The general rule we use for all covalently bound silica based supports is ~ 2,000 injections.

How do I clear a blocked HPLC column?

First, wash the column with varying solvents at a low flow rate. However, if your column is so clogged that it can’t flow at all, flip the column and run it in the opposite direction. If all else fails, then you might need a new column. Like I said earlier, columns do have a finite life.

When should I use Ace c18-ar columns?

Due to their similar hydrophobic characteristics, ACE C18-AR columns may be used for applications in which “standard” C18 columns would normally be considered. However, due to its integral phenyl functionality, ACE C18-AR is additionally recommended for separations that involve compounds containing aromatic functionality.

Why choose Ace HPLC columns?

ACE columns are available in capillary through to preparative dimensions, with a wide range of particle sizes (3, 5, 10 and 15μm), pore sizes (100Å and 300Å) and surface chemistries (C18, C8, C4, CN, Phenyl,AQ, C18-HL and SIL). Independent comparison tests show ACE HPLC columns give outstanding performance.

What are Ace c18-pfp columns used for?

Due to their similar hydrophobic characteristics, ACE C18-PFP columns may be used for applications in which “standard” C18 columns would normally be considered.

What is Ace c18-ar?

Based upon the same ultra inert, ultra pure, ultra reproducible silica platform as ACE C18, the unique ACE C18-AR phase provides an alternate selectivity to C18 columns. When should I use ACE C18-AR?