What is Staphylococcus PCR?
Background. Staphylococcus aureus is mainly acquired from hospital infections and demonstrated the ability of developing resistance to many antibiotics. Polymerase Chain Reaction (PCR) was used to identify antibiotic-resistant isolates.
What bacteria can PCR detect?
However, since the universal PCR can detect almost all bacteria, including normal flora such as staphylococci on the skin, discrimination for contaminants is difficult, particularly when specimens contain few bacteria.
What can PCR be used for?
The polymerase chain reaction has been elaborated in many ways since its introduction and is now commonly used for a wide variety of applications including genotyping, cloning, mutation detection, sequencing, microarrays, forensics, and paternity testing. Typically, a PCR is a three-step reaction.
Why is S aureus pathogenic?
aureus as a pathogen and its ability to cause such a wide range of infections are the result of its extensive virulence factors. The increase in the resistance of this virulent pathogen to antibacterial agents, coupled with its increasing prevalence as a nosocomial pathogen, is of major concern.
How does someone get Staphylococcus aureus?
Transmission. S. aureus is most often spread to others by contaminated hands. The skin and mucous membranes are usually an effective barrier against infection.
Does PCR work on bacteria?
Bacterial PCR has been successfully used for microbiological diagnosis in various infections (endocarditis, intra-amniotic infections, and meningitis) when bacterial culture has failed. 2-4 In the present work we wanted to study the value of bacterial PCR in the diagnosis of joint infection.
What does PCR allow you to do with DNA?
It is a technique used to amplify a segment of DNA of interest or produce lots and lots of copies. In other words, PCR enables you to produce millions of copies of a specific DNA sequence from an initially small sample – sometimes even a single copy.