What sites do restriction enzymes recognize?
Each restriction enzyme recognizes a short, specific sequence of nucleotide bases (the four basic chemical subunits of the linear double-stranded DNA molecule—adenine, cytosine, thymine, and guanine). These regions are called recognition sequences, or recognition sites, and are randomly distributed throughout the DNA.
Is pvu1 a restriction site in pBR322?
– BamH 1 site for the restriction enzyme is present on the gene for tetracycline resistance. Pvu 1 and Pst 1 sites are located on the ampicillin resistance gene in pBR 322.
What is the enzyme activity of sspdi (Kasi) restriction enzyme?
Thermo Scientific SspDI (KasI) restriction enzyme recognizes G^GCGCC sites and cuts best at 37°C in Tango buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Isoschizomers: BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI.
What are restricted enzymes?
Restriction enzymes recognize specific sites of different lengths and base composition. The typical restriction enzyme Type II site is an exact palindrome of 4, 5, 6, 7 or 8 base pair. For example, EcoRI recognition site is GAATTC.
What is a restriction enzyme type II site?
The typical restriction enzyme Type II site is an exact palindrome of 4, 5, 6, 7 or 8 base pair. For example, EcoRI recognition site is GAATTC. Thus, as long as the same polarity exists recognition sites generally read the same on both strands. Such sequences are often described as palindromes.
Do restricted enzymes cut methylated molecules?
Restriction enzyme will generally not cut molecules which are methylated at recognition site. Methylation takes place at other position within the recognition site may fail to affect cleavage. Restriction Enzymes Generated Staggered and Blunt Ends: